Refine your search
Collections
Journals
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Vijayabharathi, V.
- Physico-Chemical Characteristics and Antibacterial Activity of Chitosan Extracted from Shell of Crab Paratelphusa hydrodromous
Abstract Views :317 |
PDF Views:4
Authors
Affiliations
1 School of Life Science, Department of Zoology, Bharathiar University, Coimbatore, IN
2 Department of Botany, Government Arts College, Coimbatore, IN
1 School of Life Science, Department of Zoology, Bharathiar University, Coimbatore, IN
2 Department of Botany, Government Arts College, Coimbatore, IN
Source
Asian Journal of Research in Pharmaceutical Sciences, Vol 4, No 3 (2014), Pagination: 125-128Abstract
In this study, chitosan was extracted from fresh water crab Paratelphusa hydrodromous shells. In order to determine physico-chemical characteristics of the extracted chitosan, the yield, water and fat binding capacities were measured. In addition, the antibacterial activity of chitosan against Yersinia ruckeri fish pathogenic bacteria was investigated in this study. The results showed that chitosan solution at 0.5 g/kg markedly inhibited the growth of Yersinia ruckeri fish pathogenic bacteria. The results of the study indicate that crab shells are a rich source of chitosan as 38.23 of the shell's dry weight is consisted of this material. Extracted chitosan were exhibited a lower molecular weight and higher water and fat binding capacities Overall, the results indicated that chitosan was a potential bactericide against bacterial fish pathogen yersinia ruckeri.Keywords
Chitosan, Paratelphusa hydrodromous, Physicochemical Characteristics, Antibacterial Activity, Yersinia ruckeri.
References
- Knorr D. Use of chitinous polymers in food-A challenge for food research and development. Food Technology. 38; 1984: 85-97.
- No HK and Meyers SP. Crawfish chitosan as a coagulant in recovery of organic compounds from seafood processing streams. Journal of Agricultural and Food Chemistry. 37(3); 1989: 580-583.
- Ruiz-Herrera J. The distribution and quantitative importance of chitin in fungi. In: Edited by R. A. A. Muzzarelli, E. R. Pariser. Proceedings of the first international conference on chitin/chitosan. MIT Sea Grant Report MITSG78-7, Index No. 78-307-Dmb Cambridge: Massachusetts Institute of Technology. 1978; pp. 11-21.
- Knorr D. Functional properties of chitin and chitosan. Journal of Food Science. 47; 1982: 593-595.
- Yen MT. Yang JH and Mau JL. Physicochemical characterization of chitin and chitosan from crab shells. Carbohydrate Polymers. 75; 2009: 15-21.
- Chung YC. Wang HL. Chen YM. and Li SL. Effect of abiotic factors on the antibacterial activity of chitosan against waterborne pathogens. Bioresource Technology. 88; 2003: 179-184.
- Devlieghere F. Vermeulen A and Debevere J. Chitosan: Antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiology. 21; 2004: 703-714.
- Anderson DP and Siwicki AK. Duration of protection against Aeromonas salmonicida in brook trout immunostimulated with glucan or chitosan by injection or immersion. The Progressive Fish-Culturist. 56; 1994: 258-261.
- Siwicki AK. Anderson DP and Rumsey GL Dietary in- take of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vetinary Immunology Immunopathology. 41; 1994: 125-139.
- Johnson EL. and Peniston. Q.P. Utilization of shellfish waste for chitin and chitosan production. In Chemistry and Biochemistry of Marine Food Products edited by Martin RE. Flick GJ. Hebard CE. Ward DR. AVI Publishing:Westport, CT. Chapter 19; 1982.
- Green JH. and Mattick JF. Fishery waste management. In Food Processing Waste Management, Edited by Green, J.H. and Kramer, A. AVI Publishing Co. Westport, CT. 1979; pp 202-227.
- Madhavan, P., and Nair, K.G.R. (1974). Utilization of prawn waste isolation of chitin and its conversion to chitosan. Fish Technology 11: 50-53.
- Black CA Methods of Soil Analysis: Part I physical and mineralogical properties. American Society of Agronomy, Madison, Wisconsin. 1965.
- Wang JC and Kinsella JE. Functional properties of novel proteins: Alfalfa leaf protein. Journal of Food Science. 41;1976: 286-292.
- Zheng LY. Zhu JF. Study on antimicrobial activity of chitosan with different molecular weights. Carbohydrate Polymer. 54; 2003: 527-530.
- Hongpattarakere T and Riyaphan O. Effect of deacetylation conditions on antimicrobial activity of chitosans prepared from carapace of black tiger shrimp (Penaeus monodon). Songklanakarin. Journal of Science and Technology. 30(1); 2008: 1-9.
- Kamil JYVA. Jeon YJ. and Shahidi F. Antioxidative activity of chitosan of different viscosity in cooked comminuted flesh of herring (Clupea harengus), Food Chemistry. 79; 2002: 69-77.
- Tajik H. Moradi M. Rohani SMR. Efrani AM and Jalali FSS. Preparation of chitosan from brine shrimp (Artemia urmiana) cyst shells and effects of different chemical processing sequences on the physicochemical and functional properties of the product. Molecules. 13; 2008: 1263-1274.
- Rout SK. Physicochemical, functional, and spectroscopic analysis of crawfish chitin and chitosan as affected by process modification. Dissertation Louisiana State University: Baton Rouge, LA, USA. 2001.
- Cho YI. No HK. Meyers SP. Physicochemical characteristics and functional properties of various commercial chitin and chitosan products. Journal of Agricultural Food Chemistry. 46; 1998:3839-3843.
- No HK. Kim S.H. Lee SH. Park NP and Prinyawiwatkul W. Stability and antibacterial activity of chitosan solutions affected by storage temperature and time. Carbohydrate Polymers. 65; 2006: 174-178.
- No HK. Lee SH. Park NY. Meyers SP. Comparison of physicochemical, binding, and antibacterial properties of chitosans prepared without and with deproteinization process. Journal of Agriculture and Food Chemistry. 51; 2003: 7659-7663.
- Parasakthi MS. Extraction of chitin from two cephalopods. M.Sc., Dissertation, Annamalai University. India. 2004; p.18.
- Ohtakara AM. Izume M. and Mitsutomi. Action of microbial chitosanases on chitosan with different degrees of deacetylation. Agricultural and Biological Chemistry. 52; 1988: 3181-3182.
- No HK. Park NY. Lee SH and Meyers SP. Antibacterial activity of chitosan and chitosan oligomers with different molecular weights. International Journal of Food Microbiology. 74; 2002: 65-72.
- El-Ghaouth A. Ponnampalam R. Castaigne F. Arul J. Chitosan coating to extend the storage life of tomatoes. Horticulture Science. 27; 1992: 1016-1018.
- Kim CH. Kim SY. Choi KS. Synthesis and antibacterial activity of water-soluble chitin derivatives. Polymer Advanced Technology. 8; 1997: 319-325.
- Papineau AM. Hoover DG. Knorr D. Farkas DF. Antimicrobial effect of water-soluble chitosans with high hydrostatic pressure. Food Biotechnology. 5; 1991: 45-47
- Sudarshan NR. Hoover DG and Knorr D. Antibacterial action of chitosan. Food Biotechnology. 6;1992: 257-272.
- Jeon YJ. Park PJ and Kim SK. Antimicrobial effect of chitooligosaccharides produced by bioreactor. Carbohydrate Polymer. 44; 2001: 71-76.
- Ueno K. Yamaguchi T. Sakairi N. Nishi N. Tokura S. Antimicrobial activity by fractionated chitosan oligomers. In: Domard, A., Roberts, G.A.F. and Varum, K.M., Editors. Jacques Andre, Lyon, Advances in chitin Science. 2; 1997: 156-161.
- Blood Glucose Lowering Potential of Chinese Violet (Asystasia gangetica (L.) T. Anderson) in Normal and Streptozotocin Induced Diabetic Rats
Abstract Views :329 |
PDF Views:2
Authors
Affiliations
1 Department of Biotechnology, School of Life Sciences, Karpagam University, Coimbatore-641021, Tamil Nadu, IN
2 Bioprocess Lab, Department of Botany, Government Arts College, Coimbatore, Tamil Nadu-641018, IN
1 Department of Biotechnology, School of Life Sciences, Karpagam University, Coimbatore-641021, Tamil Nadu, IN
2 Bioprocess Lab, Department of Botany, Government Arts College, Coimbatore, Tamil Nadu-641018, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 6, No 4 (2014), Pagination: 170-175Abstract
Objective: The present study was undertaken to investigate the glucose reducing level by Asystasia gangetica flowers with various doses (125, 250 and 500 mg/kg bw) in normal and streptozotocin induced diabetic rats. Methods: Acute and sub-acute toxicity of A. gangetica was evaluated in rats with various doses and its and serum parameters were analyzed. Diabetes was induced by giving streptozotocin (45 mg/kg bw) to the rats and after conformation of diabetes, A. gangetica flower ethanol extract (AGFEE) was administrated to the normal and diabetic rats for a period of 14 days. Body weight and glucose levels were determined after 7th and 14th day of the study. Glucose tolerance test was also carried out after administration of glucose at the rate of 2 g/kg bw till 14th day. Results: The LD50 of extract found to be higher than 2000 mg/kg bw and the extract did not show any significant changes in serum parameters. In blood glucose reducing activity the three doses showed significant lowering glucose levels, when compared with each doses at the dose of 250 mg/kg bw the AGFEE showed maximum activity. In diabetic control rats there was a significant elevation in blood glucose levels and reduction in body weight was produced and continued till the end of the study. No alterations were found in control and extract alone treated rats. Results showed AGFEE had exhibited significant hypoglycemic (glucose lowering) activity. Conclusion: Present in vivo model indicating that the plant extract contain active principle compound for the management of hyperglycemia.Keywords
Asystasia gangetica Flowers, Streptozotocin, Glucose Tolerance, Hyperglycemia.References
- Akah PA, Ezike AC, Nwafor SV, Okoli CO, Enwerem NM. Evaluation of the anti-asthmatic property of Asystasia gangetica leaf extracts. J Ethnopharmacol 2003; 89 (1): 25-36.
- Shanmugam S, Rajendran K, Suresh K. Traditional uses of medicinal plants among the rural people in Sivagangai district of Tamilnadu, Southern India. Asian Pac J Trop Biomed 2012; 29-34.
- Senthil Kumar M, Gurumurthi P, Janardhan K. Some medicinal plants use by Irular, the tribal people of Marudhamalai hills, Coimbatotre, Tamilnadu. Nat Prod Radiance 2006; 5 (5): 382-388.
- Yeoh HH, Wong PFM. Food value of lesser utilized tropical plants. Food Chem 1993; 46 (3): 239-241.
- Kokwaro JO. Medicinal Plants of East Africa, 2nd ed. General Printers Ltd., Kenya 1976; p. 12.
- Kameswara Rao B, Renuka SP, Rajasekhar MD, Nagaraju N, Appa Rao Ch, Antidiabetic activity of Terminalia pallida fruit in alloxan-induced diabetic rats. J Ethnopharmacol 2003; 85 (1): 169-172.
- American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2005; 28: 537-542.
- Burcelin R, Eddouks M, Maury J, Kande J, Assan R, Girard J. Excessive glucose production, rather than insulin resistance, accounts for hyperglycemia in recent-onset streptozotocindiabetic rats. Diabetologia 1995; 38: 283-290.
- Weiss RB. Streptozotocin: A review of its pharmacology, efficacy and toxicity, Cancer Tret R 1982; 66: 427-438.
- Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology, 5th Edition. Edinburgh: Churchill Livingstone, Elsevier Science Ltd; 2003, 387-392.
- Jouad H, Haloui M, Rhiouani H, El Hilaly J, Eddouks M. Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez-Boulemane). J Ethnopharmacol 2001; 77:175-182.
- Sasaki T, Matsy S, Sonae A. Effect of acetic acid concentration on the colour reaction in the o-toluidine boric acid method for blood glucose estimation. Rinsho Kagaku 1972; 1: 346-353.
- Natelson S, Scott ML, Beffa C. A rapid method for the estimation of urea in biological fluids. Am J Clin Pathol 1951; 21: 275-81.
- Owen JA, Iggo JB, Scangrett FJ, Steward IP. Determination of creatinine in plasma serum, ac critical examination. J Biochem 1954; 58: 426-437.
- Parekh AC, Jung DH. Cholesterol determination with ferric acetate and sulphuric acid-ferrous sulphate reagents. Anal Chem 1970; 42: 1423-1427.
- Wolfson WQ, Cohn A, Calvary E, Ichiba F. Clinical Practical Biochemistry, Studies in serum proteins V. A rapid procedure for the estimation of total protein, true albumin, total globulin, alpha globulin, beta globulin and gamma globulin in 1.0 ml of serum. Am J Clin Pathol 1948; 18: 723-730.
- Reitman S, Frankel SA. Colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957; 28: 56-63.
- King EJ, Armstrong AR. Determination of serum and bile phosphatase activity. Can Med Assoc J 1934; 31: 56-63.
- Parminder Nain Vipin Saini, Sunil Sharma, Jaspreet Nain. Antidiabetic and antioxidant potential of Emblica officinalis mellitus (T2DM) rats. J Ethnopharmacol 2012; 142: 65-71.
- Thierry TA, Acha AE, Paulin N, Aphrodite C, Pierre K, Tazoacha A. Subacute toxicity study of the aqueous extract from Acanthus montanus. Electron J Biol 2011; 7: 11-15.
- Lahlou S. Acute and chronic toxicity of a lyophilised aqueous extract of Tanacetum vulgare leaves in rodents. J Ethnopharmacol 2008; 117: 221-227.
- Szkudelski T. The mechanism of alloxan and streptozotocin action in beta-cells of the rat pancreas. Physiol Res 2001; 50: 536-546.
- Park MW, Ha J, Chung SH. 20(S)-ginsenoside Rg3 enhances glucose-stimulated insulin secretion and activates AMPK. Biol Pharm Bull 2008; 31(4): 748-751.
- Erejuwa OO, Sulaiman SA, Wahab MS, Salam SK, Salleh MS, Gurtu S. Antioxidant protective effect of glibenclamide and metformin in combination with honey in pancreas of streptozotocin-induced diabetic rats. Int J Mol Sci 2010; 11: 2056-2066.
- Juskiewicz J, Zdunczyk Z, Jurgoński A, Brzuzan L, Godycka- Klos I, Zary Sikorska E. Extract of green tea leaves partially attenuates streptozotocin-induced changes in antioxidant status and gastrointestinal functioning in rats. Nutrition Res J 2008; 28: 343-349.
- Swanston-Flat SK, Day C, Bailey CJ, Flatt PR. Traditional plant treatment for diabetes: studies in normal and streptozotocin diabetic mice. Diabetologia 1990; 33: 462-464.
- Chatterjea MN, Shinde R. Text Book of Medical Biochemistry. Jaypee Brothers
- Medical Publishers, New Delhi, 2002; p. 317.
- Griesmacher A, Kindhauser M, Andert SE, Schreiner W, Toma C, Knoebl P, Pietschmann P, Prager R, Schnack C, Schemthaner G, Mueller MM. Enhanced serum levels of thiobarbituric-acidreactive substances in diabetes mellitus. Am J Med 1995; 98: 469-475.
- Grover JK, Yadav S, Sats V. Medicinals plants of India with anti-diabetic potential. J Ethnopharmacol 2002; 81: 81-100.
- Nakashima M, Kimura M, Matsuura H. Isolation of pseudoprototimosaponin A III from rhizomes of Anemarrhena asphodeloides and its hypoglycemic activity in streptozotocininduced diabetic mice. J Nat Prod 1993; 56: 345-350.
- Hsu FL, Chen YC, Cheng JT. Caffeic Acid as active principle from the fruit of Xanthium strumarium to lower plasma glucose in Diabetic rats. Planta Med 2000; 66: 228-230.
- Iwu MM. Antidiabetic properties of Bridelia ferruginea leaves. Planta Med 1980; 39: 247.
- Prince PSM, Menon VP. Hypoglycemic and other related action of Tinospora codifolia ischolar_mains in alloxan rats. J Ethnopharmacol 2000; 70: 19-25.
- Andrew JK. Diabetes, Churchilli living stone, New York. 2000; 1.
- Impact of Paper Pulp Industrial Effluent on Soybean Crop
Abstract Views :191 |
PDF Views:0
Authors
Affiliations
1 Bioprocess Lab, Department of Botany, Govt. Arts College, Coimbatore, Tamil Nadu – 641018, IN
1 Bioprocess Lab, Department of Botany, Govt. Arts College, Coimbatore, Tamil Nadu – 641018, IN